【个人简介】

王可品，中国科学院大学博士，中国科学院广州生物医药与健康研究院研究员，工具猪研究组研究组长，博士生导师，国家重点研发计划项目首席科学家，中国科学院青年创新促进会优秀会员，广东省杰出青年基金获得者，中国科协青年人才托举工程入选者。主要从事新型基因编辑技术开发与优化、医用基因编辑工具猪模型培育与应用等研究。近年来，开发并优化了多种新型基因编辑工具，建立了精准高效的基因编辑大动物模型创制平台，参与培育出世界最大规模的基因编辑猪模型种群和品系，填补了多项大动物模型研究领域的空白。以通讯作者或第一作者（含共同）在Genome Biology, Nucleic Acids Research, Signal Transduction and Targeted Therapy, Nature Communications, Genome Research等杂志上发表论文19篇；申请发明专利10件，其中授权1件。承担各级科研项目10余项，其中以项目负责人身份主持国家重点研发计划项目1项和国家自然科学基金项目3项。兼任广东省遗传学会秘书长。

【学科专业及研究方向】

学科专业：细胞生物学。

研究方向：新型基因编辑工具开发及优化；医用基因编辑工具猪模型培育与应用。

【科研项目】

1.国家重点研发计划“基础科研条件与重大科学仪器设备研发”重点专项项目，心肾移植猪源供体标准化体系研究，2024-01-01至2028-12-31，在研，主持；

2.国家重点研发计划“发育编程及其代谢调节”重点专项项目，猪发育及代谢突变品系的规模化创制，2021-12-01至2026-11-31，在研，参与；

3.国家自然科学基金面上项目，基于条件性表达Cas9系统构建谱系示踪工具猪模型，2022-01-01至2025-12-31，在研，主持；

4.广东省自然科学基金杰出青年项目，基于条件性表达Cas9系统构建新一代原发性胰腺导管腺癌猪模型，2025-01-01至2028-12-31，在研，主持；

5.国家自然科学基金“人类疾病大动物模型构建”专项项目，利用单碱基编辑工具构建儿童早衰症猪模型，2020-01-01至2022-12-31，结题，主持；

6.国家自然科学基金青年科学基金项目，利用CRISPR/Cpf1对杜氏肌营养不良症(DMD)猪模型进行基因治疗研究，2018-01-01至2020-12-31，结题，主持；

7.中国科学院战略生物资源能力建设项目，基因修饰猪模型资源库建设，2020-01-01至2021-12-31，结题，主持。

【代表性论文及著作】

1.Weikai Ge^#, Shixue Gou^#, Xiaozhu Zhao^#, Qin Jin^#, Zhenpeng Zhuang, Yu Zhao, Yanhui Liang, Zhen Ouyang, Xiaoyi Liu, Fangbing Chen, Hui Shi, Haizhao Yan, Han Wu*, Liangxue Lai*, Kepin Wang*, In vivo evaluation of guide-free Cas9-induced safety risks in a pig model, Signal Transduction and Targeted Therapy, 2024, 9:184.

2.Hui Shi^#, Lei Li^#, Shuangshuang Mu, Shixue Gou, Xiaoyi Liu, Fangbing Chen, Menglong Chen, Qin Jin*, Liangxue Lai*, Kepin Wang*, Exonuclease editor promotes precision of gene editing in mammalian cells, BMC Biology, 2024, 22:119.

3.Shuangshuang Mu^#, Huangyao Chen^#, Qianru Li^#, Shixue Gou^#, Xiaoyi Liu, Junwei Wang, Wei Zheng, Menglong Chen, Qin Jin*, Liangxue Lai*, Kepin Wang*, Hui Shi*, Enhancing prime editor flexibility with coiled-coil heterodimers, Genome Biology, 2024, 25:108.

4.Weikai Ge^#, Xiaozhu Zhao^#, Shixue Gou^#, Qin Jin^#, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, Kexin Zhong, Yanhui Liang, Tao Chen, Han Wu, Nan Li, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos, Molecular Therapy-Nucleic Acids, 2023, 34:102035.

5.Qin Jin^#, Hui Shi^#, Xiaoyi Liu, Jialu Ju, Kepin Wang*, Liangxue Lai*, Genome editing pig models with elements for controllable gene expression, Animal Research and One Health, 2023, 1(2):242-258.

6.Jingke Xie^#, Zhenpeng Zhuang^#, Shixue Gou^#, Quanjun Zhang, Xia Wang, Ting Lan, Meng Lian, Nan Li, Yanhui Liang, Zhen Ouyang, Yinghua Ye, Han Wu, Liangxue Lai*, Kepin Wang*, Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription, Nucleic Acids Research, 2023, 51(18):10075-10093.

7.Qin Jin^#, Xiaoyi Liu^#, Zhenpeng Zhuang^#, Jiayuan Huang^#, Shixue Gou, Hui Shi, Yu Zhao, Zhen Ouyang, Zhaoming Liu, Lei Li, Junjie Mao, Weikai Ge, Fangbing Chen, Manya Yu, Yezhi Guan, Yinghua Ye, Chengcheng Tang, Ren Huang, Kepin Wang*, Liangxue Lai*, Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation, Genome Biology, 2023, 24:8.

8.Xunlei Wang^#, Qin Jin^#, Wenwen Xiao^#, Puxian Fang, Liangxue Lai, Shaobo Xiao, Kepin Wang*, Liurong Fang*, Genome-wide CRISPR/Cas9 screen reveals a role for SLC35A1 in the adsorption of porcine deltacoronavirus, Journal of Virology, 2022, 96(24):e0162622.

9.Qin Jin^#, Xiaoyu Yang, Shixue Gou, Xiaoyi Liu, Zhenpeng Zhuang, Yanhui Liang, Hui Shi, Jiayuan Huang, Han Wu, Yu Zhao, Zhen Ouyang, Quanjun Zhang, Zhaoming Liu, Fangbing Chen, Weikai Ge, Jingke Xie, Nan Li, Chengdan Lai, Xiaozhu Zhao, Jiaowei Wang, Meng Lian, Lei Li, Longquan Quan, Yinghua Ye, Liangxue Lai*, Kepin Wang*, Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression, Science China Life Sciences, 2022, 65(11):2269-2286.

10.Yanhui Liang^#, Jingke Xie^#, Quanjun Zhang^#, Xiaomin Wang^#, Shixue Gou, Lihui Lin, Tao Chen, Weikai Ge, Zhenpeng Zhuang, Meng Lian, Fangbing Chen, Nan Li, Zhen Ouyang, Chengdan Lai, Xiaoyi Liu, Lei Li, Yinghua Ye, Han Wu, Kepin Wang*, Liangxue Lai*, AGBE: A dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns, Nucleic Acids Research, 2022, 50(9):5384-5399.

11.Nan Li^#, Shixue Gou^#, Jiaowei Wang^#, Quanjun Zhang, Xingyun Huang, Jingke Xie, Li Li, Qin Jin, Zhen Ouyang, Fangbing Chen, Weikai Ge, Hui Shi, Yanhui Liang, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Yinghua Ye, Longquan Quan, Han Wu*, Liangxue Lai*, Kepin Wang*, CRISPR/Cas9-mediated gene correction in newborn rabbits with hereditary tyrosinemia type I, Molecular Therapy, 2021, 29(3):1001-1015.

12.Meng Lian^#, Fangbing Chen^#, Xingyun Huang^#, Xiaozhu Zhao, Shixue Gou, Nan Li, Qin Jin, Hui Shi, Yanhui Liang, Jingke Xie, Weikai Ge, Zhenpeng Zhuang, Jiaowei Wang, Yinghua Ye, Yi Yang, Kepin Wang*, Liangxue Lai*, Han Wu*, Improving Cpf1-mediated base editing system by combining dCas9/ dead sgRNA with human APOBEC3A variants, Journal of Genetics and Genomics, 2021, 48(1):92-95.

13.Wenjing Li^#, Lei Shi^#, Zhenpeng Zhuang^#, Han Wu^#, Meng Lian, Yihui Chen, Lei Li, Weikai Ge, Qin Jin, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Zhen Ouyang, Yinghua Ye, Yingying Li, Hai Wang, Yuan Liao, Longquan Quan, Lei Xiao, Liangxue Lai*, Guangxun Meng*, Kepin Wang*, Engineered pigs carrying a gain-of-function NLRP3 homozygous mutation can survive to adult and accurately recapitulate human systemic spontaneous inflammatory responses, Journal of Immunology, 2020, 205(9):2532-2544.

14.Jingke Xie^#, Xingyun Huang^#, Xia Wang, Shixue Gou, Yanhui Liang, Fangbing Chen, Nan Li, Zhen Ouyang, Quanjun Zhang, Weikai Ge, Qin Jin, Hui Shi, Zhenpeng Zhuang, Xiaozhu Zhao, Meng Lian, Jiaowei Wang, Yinghua Ye, Longquan Quan, Han Wu, Kepin Wang*, Liangxue Lai*, ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems, BMC Biology, 2020, 18:131.

15.Jingke Xie^#, Weikai Ge^#, Nan Li^#, Qishuai Liu^#, Fangbing Chen, Xiaoyu Yang, Xingyun Huang, Zhen Ouyang, Quanjun Zhang, Yu Zhao, Zhaoming Liu, Shixue Gou, Han Wu, Chengdan Lai, Nana Fan, Qin Jin, Hui Shi, Yanhui Liang, Ting Lan, Longquan Quan, Xiaoping Li, Kepin Wang*, Liangxue Lai*, Efficient base editing for multiple genes/loci in pigs using base editors, Nature Communications, 2019, 10:2852.

16.Han Wu^#, Qishuai Liu^#, Hui Shi, Jingke Xie, Quanjun Zhang, Zhen Ouyang, Nan Li, Yi Yang, Zhaoming Liu, Yu Zhao, Chengdan Lai, Degong Ruan, Jiangyun Peng, Weikai Ge, Fangbing Chen, Nana Fan, Qin Jin, Yanhui Liang, Ting Lan, Xiaoyu Yang, Xiaoshan Wang, Zhiyong Lei, Pieter A. Doevendans, Joost P. G. Sluijter, Kepin Wang*, Xiaoping Li*, Liangxue Lai*, Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems, Cellular and Molecular Life Sciences, 2018, 75(19):3593-3607.

17.Degong Ruan^#, Jiangyun Peng^#, Xiaoshan Wang^#, Zhen Ouyang^#, Yi Yang, Fangbing Chen, Weikai Ge, Han Wu, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Chengdan Lai, Nana Fan, Zhiwei Zhou, Qishuai Liu, Nan Li, Qin Jin, Hui Shi, Jingke Xie, Xiaoyu Yang, Hong Song, Kepin Wang*, Xiaoping Li*, Liangxue Lai*, Xist derepression in active X chromosome hinders pig somatic cell nuclear transfer, Stem Cell Reports, 2018, 10(2): 494-508.

18.Kepin Wang^#, Qin Jin^#, Degong Ruan^#, Yi Yang, Qishuai Liu, Han Wu, Zhiwei Zhou, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Jiangyun Peng, Chengdan Lai, Nana Fan, Yanhui Liang, Ting Lan, Nan Li, Xiaoshan Wang, Xinlu Wang, Yong Fan, Pieter A. Doevendans, Joost P.G. Sluijter, Pentao Liu, Xiaoping Li*, Liangxue Lai*, Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing, Genome Research, 2017, 27(12): 2061-2071.

19.Yi Yang^#, Kepin Wang^#, Han Wu, Qin Jin, Degong Ruan, Zhen Ouyang, Bentian Zhao, Zhaoming Liu, Yu Zhao, Quanjun Zhang, Nana Fan, Qishuai Liu, Shimei Guo, Lei Bu, Yong Fan, Xiaofang Sun, Xiaoping Li*, Liangxue Lai*, Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering, Journal of Molecular Cell Biology, 2016, 8(2): 174-177.

【所获荣誉和奖励】

1.广东省自然科学基金杰出青年项目（2025）

2.中国科学院青年创新促进会优秀会员（2023）

3.中国科学院广州分院2021～2022年度优秀青年科技工作者（2023）

4.中国科协青年人才托举工程项目入选者（2021）

5.中国科学院青年创新促进会会员（2019）